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tns  (PromoCell)


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    Structured Review

    PromoCell tns
    Tns, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trypsin+neutralization+solution/Trypsin+Neutralizing+Solution/bio_rxiv__64898__2026__05__06__723334-217-17-18
    Average 94 stars, based on 83 article reviews
    tns - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Neutralization:

    Article Title: Bioinspired Oligo-Urethane Nanoparticles for Delivering Exogenous C-Type Natriuretic Peptide: Synthetic Biomaterial Nanocarrier Complexes and Their Interactions with Cardiac Myofibroblasts.
    Article Snippet: In a healthy heart, cells naturally secrete C-type natriuretic peptide (CNP), a cytokine that protects against myofibroblast differentiation of cardiac fibroblasts and extracellular matrix deposition leading to fibrosis.. CNP availability during myocardial remodeling is important to prevent cardiac fibrosis, but CNP is limited after an injury because of the loss of cardiomyocytes and the activation of cardiac fibroblasts to myofibroblasts.. We hypothesized that the sustained release of exogenous CNP from oligo-urethane nanoparticles (NPs) would reduce differentiation of human cardiac fibroblasts toward a myofibrogenic phenotype.

    Article Title: Chemically defined medium for the culture of cancer stem cell (CSC) containing cell populations
    Article Snippet: .. Materials Cancer Stem Cell Medium Phosphate Buffered Saline w/o Ca++/Mg++ (PBS, PromoCell #C-40232) Detach-Kit (Trypsin-EDTA and Trypsin Neutralization Solution; PromoCell #C-41210) 6-well Suspension Culture Plates (e.g. Greiner Bio One, #657 185) .. First, 90% of the final volume cell culture grade water is placed in a mixing vessel.

    Article Title: In Vitro Evaluation of the Regenerative Potential of Autologous Platelet-Rich Fibrin (PRF) on Human Primary Periodontal Ligament Cells
    Article Snippet: .. After 2 h of incubation at 37 °C, the reaction was stopped with 2 mL of trypsin neutralization solution (TNS, PromoCell, Heidelberg, Germany). ..

    Article Title: In Vitro Evaluation of the Regenerative Potential of Autologous Platelet-Rich Fibrin (PRF) on Human Primary Periodontal Ligament Cells.
    Article Snippet: .. After 2 h of incubation at 37 ◦C, the reaction was stopped with 2 mL of trypsin neutralization solution (TNS, PromoCell, Heidelberg, Germany). ..

    Article Title: Anti-leukocyte adhesion for the mitigation of potential adverse events caused by CD3-specific binding domains
    Article Snippet: Human brain microvascular endothelial cells (HBMEC) (#1000, Sciencell Research Laboratories) or human umbilical vein endothelial cells (HUVEC) (#C-12200, Promocell) were used as endothelial cell-model. Cryopreserved HBMEC at passage 1 (>5×105 cells/ml) were first cultivated in fibronectin-coated Nunclon-treated 75 cm2 cell culture flasks (#156499, Nunc) at 37° C. and 5% CO2 in a Heraeus Cytoperm 2 (Thermo Scientific) according to the manufacturer's instructions. .. Sub-cultivation of HBMEC was done with the Detach Kit (#C-41200, Promocell) consisting of HEPES-buffered balanced salt solution (#C-40000, Promocell), trypsin-EDTA solution (0.04%/0.03%) (#C-41000, Promocell) and trypsin neutralization solution (TNS, #C-41100, Promocell). ..

    Article Title: Activation of cAMP Signaling in Response to α-Phellandrene Promotes Vascular Endothelial Growth Factor Levels and Proliferation in Human Dermal Papilla Cells.
    Article Snippet: Primary human follicle DPCs (55-year-old, Caucasian, females) isolated from the human dermis of the scalp were purchased from PromoCell (Heidelberg, Germany) and grown in DPC growth medium with supplement mix (PromoCell) and 1% penicillin-streptomycin (10,000 U/mL; Gibco; Grand Island, NE, USA) at 37 ◦C in a 5% CO2 humidified atmosphere. .. At 70% confluency, the cells were sub-cultured according to the PromoCell subcultivation protocol using the DetachKit (2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES) buffer, trypsin/ethylenediaminetetraacetic acid (EDTA), and trypsin neutralization solution; PromoCell). ..

    Article Title: Activation of cAMP Signaling in Response to α-Phellandrene Promotes Vascular Endothelial Growth Factor Levels and Proliferation in Human Dermal Papilla Cells
    Article Snippet: Primary human follicle DPCs (55-year-old, Caucasian, females) isolated from the human dermis of the scalp were purchased from PromoCell (Heidelberg, Germany) and grown in DPC growth medium with supplement mix (PromoCell) and 1% penicillin-streptomycin (10,000 U/mL; Gibco; Grand Island, NE, USA) at 37 °C in a 5% CO 2 humidified atmosphere. .. At 70% confluency, the cells were sub-cultured according to the PromoCell subcultivation protocol using the DetachKit (2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES) buffer, trypsin/ethylenediaminetetraacetic acid (EDTA), and trypsin neutralization solution; PromoCell). ..

    Centrifugation:

    Article Title: Bioinspired Oligo-Urethane Nanoparticles for Delivering Exogenous C-Type Natriuretic Peptide: Synthetic Biomaterial Nanocarrier Complexes and Their Interactions with Cardiac Myofibroblasts.
    Article Snippet: In a healthy heart, cells naturally secrete C-type natriuretic peptide (CNP), a cytokine that protects against myofibroblast differentiation of cardiac fibroblasts and extracellular matrix deposition leading to fibrosis.. CNP availability during myocardial remodeling is important to prevent cardiac fibrosis, but CNP is limited after an injury because of the loss of cardiomyocytes and the activation of cardiac fibroblasts to myofibroblasts.. We hypothesized that the sustained release of exogenous CNP from oligo-urethane nanoparticles (NPs) would reduce differentiation of human cardiac fibroblasts toward a myofibrogenic phenotype.

    Saline:

    Article Title: Chemically defined medium for the culture of cancer stem cell (CSC) containing cell populations
    Article Snippet: .. Materials Cancer Stem Cell Medium Phosphate Buffered Saline w/o Ca++/Mg++ (PBS, PromoCell #C-40232) Detach-Kit (Trypsin-EDTA and Trypsin Neutralization Solution; PromoCell #C-41210) 6-well Suspension Culture Plates (e.g. Greiner Bio One, #657 185) .. First, 90% of the final volume cell culture grade water is placed in a mixing vessel.

    Suspension:

    Article Title: Chemically defined medium for the culture of cancer stem cell (CSC) containing cell populations
    Article Snippet: .. Materials Cancer Stem Cell Medium Phosphate Buffered Saline w/o Ca++/Mg++ (PBS, PromoCell #C-40232) Detach-Kit (Trypsin-EDTA and Trypsin Neutralization Solution; PromoCell #C-41210) 6-well Suspension Culture Plates (e.g. Greiner Bio One, #657 185) .. First, 90% of the final volume cell culture grade water is placed in a mixing vessel.

    Incubation:

    Article Title: In Vitro Evaluation of the Regenerative Potential of Autologous Platelet-Rich Fibrin (PRF) on Human Primary Periodontal Ligament Cells
    Article Snippet: .. After 2 h of incubation at 37 °C, the reaction was stopped with 2 mL of trypsin neutralization solution (TNS, PromoCell, Heidelberg, Germany). ..

    Article Title: In Vitro Evaluation of the Regenerative Potential of Autologous Platelet-Rich Fibrin (PRF) on Human Primary Periodontal Ligament Cells.
    Article Snippet: .. After 2 h of incubation at 37 ◦C, the reaction was stopped with 2 mL of trypsin neutralization solution (TNS, PromoCell, Heidelberg, Germany). ..



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